Screening of Novel Bacteriophage Infection in Pseudomonas putida Isolated From Potato Disease

Background: in recent decades bacteriophages have been used as treating agentsagainst some pathogens. also, bacteriophages could be considered as alternatives of antimicrobialagents in plant protection.objectives: this study aimed to isolate a pathogen from potato (solanum tubersom) tubers(pseudomonas putida), and of its specific bacteriophage from soil and wastewater.materials and methods: infected samples of soft rot potato tubers were collected fromflavarjan farms (isfahan province, iran) to isolate and identify p. putida by biochemicaland molecular methods. soil and wastewater samples were obtained locally to isolatethe bacteriophage attributed to p. putida. soil suspension was centrifuged and then filtratedby 0.2 micrometer millipore filter. the wastewater was directly filtrated by 0.2 micrometerfilter after centrifugation. after incubation of isolated bacteria together withphage contained solution, plaques were detected in nutrient agar. subsequently, clearanceof p. putida liquid culture incubated by added filtrated bacteriophage was observed.the structure and morphological characteristics of p. putida related bacteriophage wasremarked by transmission electron microscopy (tem).results: isolated strain sda2 was identified as pseudomonas putida with related accessionnumber hq423667. the result showed that isolated bacteriophage belonged to cystoviridaefamily.conclusions: we isolated a new strain of p. putida as well as a novel bacteriophagethrough which potato disease caused by the bacterium could be treated.