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report on the misidentification of vibrio cholera by vitek mass spectrometer
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نویسنده
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cai shumei ,liu yichu ,chen juan ,zhang shenghang
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منبع
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jundishapur journal of microbiology - 2023 - دوره : 16 - شماره : 11 - صفحه:1 -6
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چکیده
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Introduction: vibrio cholera is the primary culprit of the cholera epidemic, causing gastrointestinal diseases. non-o1/o139 strains of v. cholera spread into food and drink, causing food poisoning. the present study aimed to investigate the identification ability of the vitek mass spectrometer (vitek ms) for v. cholera strains.case presentation: firstly, we utilized vitek ms and vitek 2 compact, in addition to the serum agglutination test and 16s rrna gene sequencing, to identify two v. cholera strains. the vitek ms was employed to identify two clinical strains of v. cholera, and their protein spectra were compared. the vitek ms incorrectly identified one strain as vibrio mimicus with a confidence level of 99.9%; nevertheless, another strain was correctly identified as v. cholera with a confidence level of 99.9%. vitek 2 compact identified both strains as v. cholera at a confidence level of 97.0%. serological tests revealed that the v. cholera strains were neither in the o1 nor o139 group. the 16s rrna sequencing results confirmed the strains as v. cholera. the protein profiles of v. cholera and v. mimicus were observed to be similar, leading to the inability of the mass spectrometer to distinguish between them.conclusions: vibrio mimicus and v. cholera both belong to the vibrio genus. the vitek ms cannot completely differentiate between v. cholera and v. mimicus. for accurate identification, it is recommended to use biochemical serological tests by vitek 2 compact or serum.
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کلیدواژه
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vibrio cholera ,mass spectrometer ,gene sequencing ,serum agglutination
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آدرس
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fujian medical university, fuzhou general clinical medical school (the 900th hospital), department of clinical laboratory medicine, china. xiamen university, affiliated dongfang hospital of school of medicine, fujian key laboratory of aptamers technology, china, fujian medical university, fuzhou general clinical medical school (the 900th hospital), department of clinical laboratory medicine, china. xiamen university, affiliated dongfang hospital of school of medicine, fujian key laboratory of aptamers technology, china, fujian medical university, fuzhou general clinical medical school (the 900th, department of clinical laboratory medicine, china, fujian medical university, fuzhou general clinical medical school (the 900th hospital), department of clinical laboratory medicine, china. xiamen university, affiliated dongfang hospital of school of medicine, fujian key laboratory of aptamers technology, china
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پست الکترونیکی
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fzzyyzsh@126.com
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Authors
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