extended-spectrum beta-lactamases genes in klebsiella pneumoniaeisolates obtained from patients in intensive care units

Background: drug-resistant hospital-acquired infections (hais) are a growing concern in modern medicine throughout the world. klebsiella pneumoniaeis one of the most prominent causative agents of multidrug-resistant nosocomial infections. it is also widely recognized for having a high resistance level to many antibiotic classes, particularly beta-lactams. carbapenemase-producing k. pneumoniaehas been identified as a major global cause of hais with adverse clinical outcomes. therefore, it is of the utmost importance to have an in-depth understanding of the antimicrobial resistance (amr) genetic determinants of this bacterium to stop the spread of highly resistant k. pneumoniaein healthcare facilities and the resulting patient morbidity and mortality. objectives: this study aimed to investigate the amr pattern of k. pneumoniaeisolates obtained from intensive care units (icus), with a focus on extended-spectrum beta-lactamases (esbls) genes blactx-m, blages, and blaimp. methods: a total of 105 k. pneumoniaeisolates obtained from the sputum samples of icu patients were identified and confirmed using standard microbiological tests and 16s rrna polymerase chain reaction (pcr). the antibiotic susceptibility test was performed for all the isolates. the presence of esbl genes was determined phenotypically and by pcr. results: the highest level of resistance was observed against ceftazidime (100%), cefotaxime (99%), and imipenem (93.3%). approximately 87.6% and 39% of the isolates were sensitive to colistin and gentamicin, respectively. phenotypic esbl production was observed in 16 isolates, and the prevalence of blactx-mwas 86.7%. no blagesand blaimpgenes were detected. conclusions: periodic investigation of amr-mediating genes is essential due to the high prevalence of esbl genes in hais. the presence of other esbl genes needs to be investigated for a more accurate understanding of the amr status of k. pneumoniaein healthcare settings.