isolation and analysis of the biological characteristics of a novel bacteriophage vb_saup_p992 against <i>staphylococcus aureus</i>

Background: staphylococcus aureus is one of the most virulent pathogens inducing various diseases in humans and animals. disturbingly, the degree and rate of drug resistance in this pathogen have sharply increased and have become a global concern. objectives: this study analyzed the lytic activity and the biological characteristics of a mitomycin c-induced bacteriophage from s. aureus isolated and identified from hospital sewage to explore novel antibacterial therapeutic strategies for the clinical treatment of drug-resistant s. aureus, including urinary tract infections caused by mrsa strains. methods: the new bacteriophage vb_saup_p992, which can effectively lyse the mrsa strain, was successfully isolated and purified using the double agar plate method. in this regard,phsensitivity, one-step growth curve, the optimal multiplicity of infection (moi), thermo-sensitivity, phage host range, and the effects of organic reagents on phage activity were determined. results: electron microscopic results showed that the bacteriophage head was hexagonal with a non-contractile tail and could form a single, neatly-bordered plaque. moreover, the optimal moi was 0.1. the one-step growth curve showed a bacteriophage incubation period of about 20 min, a lysis period of 90 min, and a burst size of about 65.8 pfu per infected cell. the bacteriophage vb_saup_- p992 had acceptable thermal stability, ph stability, and resistance to physical and chemical factors, indicating a bacteriophage with no capsule. conclusions: with an intense lytic activity and acceptable stability, this novel bacteriophage lays a solid foundation to enrich the bacteriophage library and better prevent and control drug-resistant s. aureus infections.