genetic cassettes profiling of class i integron and antimicrobial susceptibility profiles among pseudomonas aeruginosa isolates collected from patients in north of iran

Background: pseudomonas aeruginosa is an opportunistic nosocomial bacterium, especially in infection wards and among patients with burns. the present study addressed the molecular investigation of the gene cassettes of class i integrin (inti) and its relationship with multiple drug resistance in clinical samples of p. aeruginosa isolated from babol hospitals in north of iran. objectives: this study aimed to detect the frequency of inti and gene cassettes in the clinical isolates of p. aeruginosa. methods: in this study, from 75 clinical samples, 30 strains were identified using specific biochemical methods. after determining antibiotic susceptibility using disk diffusion and agar dilution, the frequency of the inti gene and its gene cassettes were determined using the polymerase chain reaction (pcr) method. results: the highest resistance rate was observed for cefotaxime, ampicillin, and nitrofurantoin using disk diffusion and agar dilution methods. the molecular analyses revealed that 60% of the isolates had the inti genes. the frequency of the aadb, dfra1, and bla-oxa30 genes were 61, 66, and 33%, respectively. conclusions: the high resistance of pseudomonas isolates is due to the presence of inti and its gene cassettes. considering their high resistance to cefotaxime, gentamicin, ampicillin, and imipenem in hospitals, selecting appropriate drugs or generally changing the treatment course for patients is possible to prevent the spread of resistance inducing genes and the development of nosocomial infections.