reverse transcription loop-mediated isothermal amplification assay with high sensitivity to rapid detection of viable salmonella in foods

Background: salmonella is one of the main foodborne bacterial pathogens, causing diseases and death. the study used reverse transcription loop-mediated isothermal amplification (rt-lamp) to detect salmonella. objectives: to design six primers and detect salmonella using rt-lamp to facilitate the rapid detection of pathogenic bacteria in food. methods: we designed six primers based on the gene coding sequences of inv a, specific to salmonella. each reaction solution contained 6.0 mm mgso4, 1 m betaine, 1.6 mm dntps, 160 u/ml bst dna polymerase, 0.2 µm of both external primers, 0.8 µm of both internal primers, and 0.2 µmof both loop primers. the reaction temperature was 65°c. results: our amplified products were separated by 2% agarose gel electrophoresis. the detection limit was 10 cfu per reaction. conclusions: rt-lamp exhibited the same accuracy as the chinese national standard assay (gb 4789.30-2016) assay in detecting salmonella in foods. rt-lamp was highly specific and sensitive; hence, it may serve as an effective tool in detecting salmonella.