molecular characteristics of carbapenem-resistant klebsiella pneumoniae isolates producing blavim, blandm, and blaimp in clinical centers in isfahan, iran

Background: the emergence and spread of metallo-beta-lactamase (mbl)-producing klebsiella pneumoniae are growing global public health concerns. one of the most common mechanisms of carbapenem resistance is the production of mbls, including verona integron-encoded metallo-beta-lactamase (vim), new delhi metallo-beta-lactamase (ndm) and imipenemase (imp). objectives: this study aimed to investigate mbls production among k. pneumoniae isolates. methods: in this study, 240 k. pneumoniae isolates were collected from clinical samples in three clinical centers of isfahan, iran, during february 2017 and november 2018. all isolates were identified using biochemical, microbiological, and molecular methods, and then antimicrobial susceptibility tests were performed to find mbl-producing isolates via phenotypic and genotypic detection methods. moreover, the minimum inhibitory concentration (mic) of antibiotics against mbl-positive strains was determined by etest. eventually, the clonal relatedness of the mbl-positive strains was analyzed using both multilocus sequence typing (mlst) and rep-pcr. results: overall, 33.7% (81/240) of the isolates were resistant to carbapenems, amongwhich 25 (30.8%) were considered mbl-positive. among 81 strains resistant to carbapenems, genes encoding fimh, rmpa, and mrkd were detected in 87.6% (71/81), 11.1% (9/81), and 67.9% (55/81) of the isolates, respectively. besides, tem and shv as antibiotic resistance genes were detected in 49.3% (40/81) and 80.2% (65/81) of the isolates. but, maga was not detected in any of the tested isolates. the pcr results revealed that blavim-1 was the most prevalent gene (13.6%; 11/81), while both blaimp-1 and blandm-1 were only detected in two isolates. multilocus sequence typing demonstrated that 15 mbl producers belonged to three sequence types (st): 11 to st23, two to st1147, and two to st15. finally, rep-pcr typing showed similar fingerprints with mlst, except for st23, such that st23 was discriminated in two clonal groups, suggesting the greater discriminatory power of rep-pcr. conclusions: here, we reported the emergence of mbl-producing k. pneumoniae in clinical centers of isfahan, iran. the findings are alarming and represent the urgent need for the application of infection control programs.