adhesin hpaa of helicobacter pylori promoted migration of ags cells via il-21 secretion from hpaa-induced cd4+t cells

Background: as known, there is a high correlation between helicobacter pylori infection and gastric carcinoma. objectives: concerning the important role of adhesin hpaa of h. pylori in the infection process, we aimed to explore whether hpaa promotes gastric cancer metastasis. methods: in this study, the levels of il-21, mmp-2, and mmp-9 in patients’ biopsies with h. pylori infection were compared with posttreatment condition. the levels of il-21 from cd4+ t cells and culture supernatants with the recombinant hpaa treatment were detected, and then the levels of mmp-2, mmp-9, and metastasis were detected and verified via ags cells co-cultured with aforesaid cd4+ t cells. results: our results showed that higher levels of il-21, mmp-2, and mmp-9 in patients’ biopsies with h. pylori infection than without h. pylori infection. adhesin hpaa induced more il-21 via cd4+ t cells, and il-21 induced high mmp-2 and mmp-9 via ags cells. in particular, hpaa caused this serial reaction to improve the migration of ags cells, and aptamer ha6 (our previous report) and anti- il-21 mcab reduced the above phenomenon remarkably. conclusions: in summary, our research suggested that adhesin hpaa plays a significant role in the process of gastric carcinoma cell metastasis via il-21 from hpaa- induced t cells, and aptamer ha6 may be a potential therapeutic agent for h. pylori treatment.