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effects of lmo2672 deficiency on environmental adaptability, biofilm formation, and motility of listeria monocytogenes
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نویسنده
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li jie ,qiao mengfan ,zhang xingxing ,li jing ,meng qingling ,qiao jun ,li yan ,wang xiaoting ,zhang guowu ,zhang kai ,wang lixia ,cai xuepeng
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منبع
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jundishapur journal of microbiology - 2020 - دوره : 13 - شماره : 5 - صفحه:1 -0
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چکیده
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Background: listeria monocytogenes is a food-borne pathogen with strong environmental adaptability. it can survive at high temperaturesand in acidic high-salt and other unfavorable stressful environments and consequently form a biofilm.objectives: this study aimed to explore the roles of lmo2672 on the environmental adaptability, biofilm formation, and motility ofl. monocytogenes.methods: we analyzed the molecular characteristics of lmo2672 protein, constructed a lmo2672 gene deletion strain of l. monocytogenesstrain (l. monocytogenes-∆lmo2672), using the homologous recombination technique and compared the environmentaladaptability, biofilm formation, and motility of l. monocytogenes-∆lmo2672 with its parental strain l. monocytogenes egd-e. the impactsof lmo2672genedeficiencyonthe transcription of genes associated with flagella formationandtheenvironmental adaptabilityof l. monocytogenes were also determined.results: the results showed that the lmo2672 contains two helix-turn-helix (hth) dna-binding domains. compared to l. monocytogenesegd-e, l. monocytogenes-∆lmo2672 grew significantly slower at different temperatures (37°c and 42°c) under different osmoticpressures (5% and 8% nacl) and in different media containing 0.3% bile salts, 5 mm h2o2, or 1% triton x-100 (p < 0.05). moreover,lm-∆lmo2672 showed a significant decrease in biofilm formation (p < 0.01) and motility (p < 0.05) at 48 h of culture. furthermore,the transcriptional levels of regulatory gene prfa and flagella-related genes mota, flip and flie significantly decreased in lm-∆lmo2672(p < 0.05).conclusions: in general, these findings indicated that lmo2672 played critical regulatory roles in the environmental adaptability,biofilm formation, and motility of l. monocytogenes, thus providing new insights into the regulatory mechanism of lmo2672 in l.monocytogenes.
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کلیدواژه
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listeria monocytogenes ,arac ,lmo2672 ,biofilm ,environmental adaptability ,motility
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آدرس
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shihezi university, college of animal science and technology, china, shihezi university, college of animal science and technology, china. central south university, school of biological engineering, china, shihezi university, college of animal science and technology, china. xinjiang academy of agricultural and reclamation science, state key laboratory for sheep genetic improvement and healthy production, china, shihezi university, college of animal science and technology, china, shihezi university, college of animal science and technology, china, shihezi university, college of animal science and technology, china, shihezi university, college of animal science and technology, china, shihezi university, college of animal science and technology, china, shihezi university, college of animal science and technology, china, shihezi university, college of animal science and technology, china, shihezi university, college of animal science and technology, china, chinese academy of agricultural sciences, lanzhou veterinary research institute, state key laboratory of veterinary etiological biology, china
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پست الکترونیکی
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944900586@qq.com
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Authors
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