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evaluation of mrna expression levels of cyp51a and mdr1, candidate genes for voriconazole resistance in aspergillus flavus
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نویسنده
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fattahi azam ,zaini farideh ,kordbacheh parivash ,rezaie sasan ,safara mahin ,fateh roohollah ,farahyar shirin ,kanani ali ,heidari mansour
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منبع
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jundishapur journal of microbiology - 2015 - دوره : 8 - شماره : 12 - صفحه:1 -6
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چکیده
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Background: voriconazole resistance (vrc-r) in aspergillus flavus isolates impacts the management of aspergillosis, since azoles are the first choice for prophylaxis and therapy. however, to the best of our knowledge, the mechanisms underlying voriconazole resistance are poorly understood.objectives: the present study was designed to evaluate mrna expression levels of cyp51a and mdr1 genes in voriconazole resistant a. flavus by a real-time reverse transcriptase polymerase chain reaction (rt-pcr) technique.materials and methods: five a. flavus isolates with resistance to vrc were examined by a rt-pcr approach.results: four out of five isolates revealed cyp51a and mdr1 mrna overexpression. interestingly, the isolate, which was negative for cyp51a and mdr1 mrna expression showed a high voriconazole minimum inhibitory concentration (mic). furthermore, a computational-based analysis predicted that voriconazole resistance could be mediated through cooperation with a network protein interaction.conclusions: our experimental and in silico findings may provide new insight in the complex molecular pathways of drug resistance and also could assist design an efficient therapeutic strategy for aspergillosis treatment.
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کلیدواژه
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drug resistance ,minimum inhibitory concentration ,cyp51a ,mdr1
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آدرس
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tehran university of medical sciences, school of public health, department of medical mycology and parasitology, iran, tehran university of medical sciences, school of public health, department of medical mycology and parasitology, iran, tehran university of medical sciences, school of public health, department of medical mycology and parasitology, iran, tehran university of medical sciences, school of public health, department of medical mycology and parasitology, iran, tehran university of medical sciences, school of public health, department of medical mycology and parasitology, iran, qom university of medical sciences, faculty of medicine, department of microbiology and immunology, iran, iran university of medical sciences, faculty of medicine, department of medical mycology and parasitology, iran, tehran university of medical sciences, school of public health, department of medical mycology and parasitology, iran, tehran university of medical sciences, exprerimental medicine research center, department of medical genetics, iran
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پست الکترونیکی
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mheidari@sina.tums.ac.ir
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Authors
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