levels of transforming growth factor-beta after immunization of mice with in vivo prepared toxoplasma gondii excretory/secretory proteins

Background: zoonotic parasite toxoplasma gondii has a high prevalence in human populations. a suitable vaccine for animals can stop the transmission of infection between animal and human. objectives: the aim of this study was to evaluate in vivo prepared excretory/secretory antigens (e/sa) as a potential candidate for immunization against the parasite and its effect on the production of transforming growth factor-beta (tgf-β). materials and methods: toxoplasma gondii tachyzoites were inoculated in the peritoneal cavity of mice and e/sa was harvested and used in animal immunization with and without adjuvant. serum levels of anti-e/sa antibodies and tgf-β were measured in days 0, 3, 7, 14, 28 and 56 after immunization using elisa technique. the measurements were statistically analyzed. results: our results showed that the serum levels of anti-e/sa immunoglobulins significantly increased in all of the immunized groups. the differences of the serum levels of tgf-β between the groups were statistically significant at days 28 and 56 after immunization with e/sa. conclusions: based on our study, in vivo prepared e/sa may be considered as a good candidate for animal immunization.