construction of a dna vaccine encoding mtb32c and hbha genes of mycobacterium tuberculosis

Background: tuberculosis (tb) is a contagious disease caused by mycobacterium tuberculosis. development of a new vaccine for tuberculosis requires immunogenic antigens capable of inducing suitable and long-lasting t cell immunity. the emergence of multidrugs and extensively drug-resistant strains of m. tuberculosis has made it a global public health concern. objectives: dna vaccine is a straightforward method to introduce antigens to the host. in the present study, two immunodominant mycobacterial antigens (mtb32c and hbha) were isolated and cloned into pcdna3.1 (+) to design and construct a new dna vaccine. this vector is capable of producing new potent chimeric protein. materials and methods: mtb32c (rv0125) and heparin-binding haemagglutinin adhesion (hbha) genes were amplified using polymerase chain reaction (pcr) of m. tuberculosis h37rv genome and ligated into pcdna3.1 (+). colony-pcr and restriction enzyme analysis were used to confirm the accuracy of the cloning procedure. results: in the current study, recombinant pcdna3.1 (+) vector containing mtb32c and hbha genes was successfully constructed. the desired size of dna fragment was observed using single and double digestion methods. conclusions: mtb32c and hbha genes were successfully isolated from h37rv genome and cloned into an eukaryotic expression vector. this vector can be considered as a vaccine to evaluate immune responses in animal models.