frequency assessment of oxa-10 and per β-lactamase genes and determination of minimum inhibitory concentration in klebsiella strains isolated from urinary tract infections

Background: klebsiella is one of the enterobacteriaceae family that causes infections such as pneumonia, urinary tract infections (uti), and meningitis. klebsiella strains are capable of producing enzymes that can degrade the thirdgeneration of cephalosporins known as broadspectrum betalactamase enzymes. the resistance of klebsiella strains to β-lactam antibiotics is related to the presence of β-lactamase genes. methods:in this study, 90 isolates of klebsiella were isolated from two inpatient and outpatient groups, each of them was 45 isolates, which were collected from patients with urinary tract infection in educational hospitals of shahrekord. the isolates were identified using phenotypic agar diffusion, disc phenotypic confirmation tests, and etest of extendedspectrum betalactamase (esbl). the pcr molecular method was used to diagnose and determine the strains containing broadspectrum β-lactamases. results:thirty (66%) inpatients and 8 (17.8%) outpatients had broadspectrum β-lactamase enzymes. the frequency of β-lactamase oxa10 genes and per in inpatients were 90% and 33%, respectively and also in outpatients were 50% and 12.5%, respectively. conclusions:this study showed that the prevalence of isolated klebsiella producing broadspectrum β-lactamases is higher in inpatients in comparison to outpatients. therefore, the rapid and accurate identification of bacteria and their resistance genes in clinical microbiology labs are highly recommended.