antibacterial activity and composition of the fermentation broth of streptomyces parvus 33

Background: microorganisms represent a potentially valuable source of biologically significant compounds that should be ex- plored for their potential agrochemical use. objectives: the compounds zm-1 and zm-2 were isolated from the extract of the fermentation broth of streptomyces parvus 33 by bioassay-guided fractionation. methods: the compound zm-1 was isolated in the form of a clear single crystal for the first time, and identified as holomycin via x-ray crystallography. the compound zm-2 was characterized by ir, 1 h-nmr, 13 c-nmr, dept (90° and 135°), and ms analyses. results: the compound zm-1 showed a strong antibacterial activity against the tested bacteria, and its value of minimum inhibitory concentration (mic) was greater than that of the positive control ampicillin. the compound zm-1 also exhibited significant antimi- crobial activities against some plant pathogenic fungi. nevertheless, zm-2 showed no activities against the tested bacteria and plant pathogenic fungi. conclusions: the compound zm-1 was isolated in the form of a clear single crystal for the first time, and identified as holomycin via x-ray crystallography. streptomyces parvus 33 is a newly discovered producer of holomycin. zm-1 showed a strong antibacterial activity against the tested bacteria. therefore, the compound zm-1 is a valuable lead compound for the development of agricultural fungicides while acts against bacteria, as well.