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   prevalence and genotyping of methicillin-resistant and - susceptible staphylococcus aureus strains isolated from patients in a university hospital, isfahan, iran  
   
نویسنده havaei asghar ,halaji mehrdad ,vidovic sinisa ,dillon jo-anne r. ,karbalaei mohsen ,ghanbari fahimeh ,ebrahimzadeh namvar amirmorteza ,nasr esfahani bahram ,havaei mehdi ,azimian amir
منبع jundishapur journal of microbiology - 2017 - دوره : 10 - شماره : 5 - صفحه:1 -8
چکیده    Background: staphylococcus aureus is one of the major causes of community- and hospital-acquired infections, with methicillinresistant strains showing the highest rates of morbidity and mortality. in our previous experiment, isolates, which were also used in the present study, were assessed using multilocus sequence typing (mlst). the sequence types (sts) were determined and documented in the corresponding database. objectives: in the current study, the isolates were subjected to genotyping with coagulase, sccmec, and agr typing methods. methods: a total of 54 isolates were evaluated by polymerase chain reaction (pcr) assay for meca gene, sccmec typing, and finally pcr-restriction fragment length polymorphism (rflp) for coagulase (coa) gene using alul enzyme. mlst of the isolates showed that the majority of methicillin-resistant s. aureus (mrsa) isolates belonged to st239. results: phenotypic and genotypic tests revealed that 21% of the isolates were mrsa. pcr-rflp test for coa gene showed similar patterns of mrsa isolates. the majority of the isolates were community-acquired and belonged to the sccmec type iv, whereas the remaining were hospital-acquired and classified as type i (22.2%) and type iii (2.2%). conclusions: most of the isolates belonged to agr type i, followed by type ii and type iii. agar dilution method showed higher sensitivity and specificity, compared to the disk diffusion method. the majority of the isolates were community-acquired and belonged to sccmec type iv and agr type i, whereas the remaining were hospital-acquired and classified as types i (22.2%), type iii (2.2%), and agr type i.
کلیدواژه mrsa ,mssa ,molecular characterization
آدرس isfahan university of medical sciences, school of medicine, nosocomial infection research center, department of microbiology, ایران, isfahan university of medical sciences, school of medicine, nosocomial infection research center, department of microbiology, ایران, university of saskatchewan, vaccine and infectious disease organization, canada, university of saskatchewan, vaccine and infectious disease organization, canada, mashhad university of medical sciences, school of medicine, antimicrobial resistance research center, ایران, islamic azad university, falavarjan branch, faculty of bioscience, department of microbiology, ایران, babol university of medical sciences, faculty of medicine, department of microbiology, ایران, isfahan university of medical sciences, school of medicine, department of microbiology, ایران, shiraz university, faculty of sciences, department of biology, ایران, north khorasan university of medical sciences, department of pathobiology and laboratory sciences, ایران
پست الکترونیکی amir_azimian2003@yahoo.com
 
     
   
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