role of hdm2 gene in radiosensitivity of esophageal cancer cell lines to irradiation

Background: radio therapy plays an important role in controlling tumor growth in esophageal cancer patients.objectives: our study provided comprehensive information about radio-sensitivity of hdm2 gene in esophageal cancer cells in cancer cells.methods: the expression of hdm2 gene in radiosensitive and radio-resistant esophageal squamous cell carcinoma te1, te8 and te11 cell lines is studied. rna extracted from each esophageal cancer cell, then quantitative reverse transcription pcr (rt-qpcr) assay were used for measuring hdm2 gene expression. finally, radiosensitivity was determined for every three esophageal cancer cell lines.results: it was found that hdm2 gene significantly expressed in te1, te8 and te11 cell lines by qrt-pcr analysis. clonogenic assay showed that te-11 was very sensitive to radiation relative to other cell lines.conclusions: hdm2 protein binding to the p53 protein is a key regulator of cell cycle control and ‎genome protection cell cycle control, programmed cell death and response cells to ‎radition play important roles.‎