Detection of Somatic mutation of codon 248 of p53 gene between Iranian women with breast cancer

Detection of p53 gene mutation on its codon 248 between iranian women withbreast cancer by pcr-rflp.material and methods: dna extraction of tumor tissues was performed in 53 patients withprimary breast cancer. designing primer for the sub-studying codon was performed with ntivectorsoftware and detection of codon 248 mutation was done by msp i pcr_rflp.results: the somatic mutations of p53 on codon 248 were observed in 11.3% (6 of 53) of ourcases, in which 9.4% were heterozygote mutation and the rest were homozygote mutation.this ratio is the highest reported percentage in the world.conclusion: this perusal indicates genotyping of p53 gene on codon 248 is important foriranian women with breast cancer risk factors.