effects of chrysin-plga-peg nanoparticles on proliferation and gene expression of mirnas in gastric cancer cell line

Background: recently, chrysin, as a flavone, has revealed cancer chemo-preventive activity. the present experiment utilized the plga-peg-chrysin complex, and free chrysin, to evaluation of the expression of mir-22, mir-34a and mir-126 in human gastric cell line. objectives: the purpose of this study was to examine whether nano encapsulating chrysin improves the anti-cancer effect of free chrysin on ags human gastric cell line. methods: properties of the chrysin encapsulated in plga-peg nanoparticles were investigated by sem, h nmr, and ftir. the assessment of cytotoxicity on the growth of the human gastric cell line was carried out through mtt assay. after treating the cells with a prearranged amount of pure and encapsulated chrysin, rna was extracted and the expressions of mir-22, mir-34a and mir-126 were measured by using real-time pcr. results: with regard to the amount of the chrysin loaded in plga-peg nanoparticles, ic50 value was significantly decreased in nanocapsulatedchrysin, in comparison with free chrysin. this finding has been proved through the further increase of mir-22, mir-34a and mir-126 gene expression of nanocapsulatedchrysin, in comparison with free chrysin. conclusions: in this study, we revealed that the plga-peg-chrysin is more effective than free chrysin in inhibiting the growth of human gastric cell line.