E-SELECTION GENE Polymorphisms in Iranian Chronic Hepatitis B Patients

Background and aims: the aim of this study was to detect the substitutions ser128arg (a128c) and leu554phe (t554c)which are responsible for e-selectin polymorphisms in patients with chronic hepatitis b and healthy controls. weinvestigated possible association of the ser128arg and leu554phe gene polymorphisms in the e-selectin gene withsusceptibility to chronic hepatitis b.methods: sixty-three patients with chronic hepatitis b virus infection and 150 healthy subjects were recruitedsequentially as they presented to clinic. classification of chronic hepatitis b virus (hbv)-infected patients was asasymptomatic carrier state (34 patients) and chronic hepatitis b (29 patients). genomic dna was isolated from anticoagulatedperipheral blood buffy coat using miller's salting-out method. the presence of the e-selectin genepolymorphisms was determined by using polymerase chain reaction amplification refractory mutation system (arms).results: distribution of e-selectin 128 (a+c-, a+c+, a-c+) genotypes and e-selectin 554 (c+t-, pc-, c+p) genotypes werenot statistically different in chronic hepatitis b patients and controls (p=0.41 and 0.96, respectively). also, two groupshad no significant difference in distribution of frequencies of allele 128a (p=0.41), 128c (p=0.15), allele 554c(p=0.85), and allele 554t (p=0.76). carrying of allele 128a (or=0.58, 95% ci=0.16-2.12), 128c (or=1.52, 95%ci=0.84-2.74), 554c (or=1.24, 95% ci=0.12-12.08), and allele 554t (or=0.88, 95% ci=0.38-2.01) were not riskfactors for susceptibility to chronic hepatitis b infection.conclusions: carrying e-selectin gene polymorphisms of ser128arg and leu554phe is not considered risk factor forsusceptibility to chronic hepatitis b infection