Intraperitoneal Transplantation of Hepatocytes Embedded in Thermoreversible Gelation Polymer (Mebiol Gel) in Acute Liver Failure Rat Model

Orthotopic liver transplantation (olt) is the only treatment that improves the survival rate in patients with acute liverfailure (alf). hepatocytes are anchorage dependent and require a substratum for their long-term survival. varioussubstrata are being used to improve the function and survival of these hepatocytes, but cannot be used clinically as theyare animal derived. this has been overcome by thermoreversible gelation polymer (tgp). in the present study, rathepatocytes embedded in tgp, were transplanted intraperitoneally in acute liver failure rat model. efficacy of thetransplanted cells was studied by assessing biochemical parameters and histopathology at different time points. thealf condition reverted to normal 15 days after cell transplantation. the transplanted animals survived 100%. thereforethis study suggests that the cells in tgp provide substratum to the transplanted cells for long term survival and couldsuccessfully provide liver support in severe alf