Potential Activity of Camel Milk-Amylase and Lactoferrin against Hepatitis C Virus Infectivity HepG2 and Lymphocytes

Background and aims: to exploring which camel milk proteins, have the ability to inhibit and/or blocking the hepatitisc virus (hcv) entry and replication inside the cells system.methods: using human peripheral blood mononuclear cells (pbmcs) and hepg2 cells system, three experiments weresetup: 1) cells treated with amylase or lactoferrin then infected with hcv; 2) hcv treated with amylase or lactoferrinthen used to infect the cells; and 3) hcv infected cells were treated with amylase or lactoferrin. rna was extracted, rtpcrand nested pcr were run, in addition to immune-staining the cells to localize the viral molecule within the cellsfoci.results: camel milk-amylase and lactoferrin were in vitro tested the ability to inhibit the hcv entry and replicationinside the human peripheral blood and hepg2. amylase could not able to inhibit nor blocking the viral replication.however, lactoferrin demonstrate a clear ability to inhibit the viral entry into both cells system when pre-interact withthe virus, but fault to protect the cells before infection. the virus replication inside the cells was completely blockedonly when the infected cells were treated with lactoferrin.conclusions: camel lactoferrin was demonstrated a remarked in vitro ability to completely inhibit the hcv entry intopbmc, hepg2 and replication inside those cells system