Effect of Proanthocyanidin, Fluoride and Casein Phosphopeptide Amorphous Calcium Phosphate Remineralizing Agents on Microhardness of Demineralized Dentin

Objectives: the aim of this study was to evaluate the effect of dentin remineralization using proanthocyanidin (pa), fluoride varnish and casein phosphopeptide amorphous calcium phosphate (cpp-acp) paste and their various combinations on microhardness of demineralized root dentin. materials and methods: one-hundred and twenty freshly extracted sound human premolars were selected and randomly divided into eight groups for dentin treatment as follows. c: deionized water (control); pa: 6.5% pa solution; f: fluoride varnish (5% naf, 22600 ppm fluoride); cp: ccp-acp; paf: 6.5% pa + fluoride varnish; pacp: 6.5% pa + ccp-acp; fcp: fluoride varnish + ccp-acp and pafcp: 6.5% pa + fluoride varnish + ccp-acp. all specimens were subjected to vickers microhardness test (500 g, 10 seconds, 3 points). data were analyzed using one-way anova and tukey’s post hoc test. the significance level was set at 0.05. results: the mean and standard deviation (sd) values of vickers hardness number (vhn) in groups c, pa, f, cp, paf, pacp, fcp and pafcp were 37.39±4.97, 38.68±4.62, 48.28±2.68 ,41.91±3.32, 48.59±2.55, 53.34±2.57, 48.413±4.00 and 55.20±1.82, respectively. pairwise comparisons of the groups revealed that there was no significant difference between groups c and pa, pa and cp, f and paf, f and fcp, paf and fcp, and pacp and fpacp (p>0.05); but significant differences were observed between other groups (p<0.05). conclusions: the results of this study showed that the tested dentin treatments increased the microhardness of demineralized root dentin except for pa application.