Cytotoxic Effects and Osteogenic Activity of Calcium Sulfate With and Without Recombinant Human Bone Morphogenetic Protein 2 and Nano-Hydroxyapatite Adjacent To Mg-63 Cell Line

Objectives: the aim of this study was to assess the cytotoxic effects and osteogenic activity of recombinant human bone morphogenetic protein (rhbmp2) and nano-hydroxyapatite (n-ha) adjacent to mg-63 cell line. materials and methods: to assess cytotoxicity, the 4,5-dimethyl thiazolyl-2,5-diphenyl tetrazolium bromide (mtt) assay was used. alkaline phosphatase (alp) activity and osteogenic activity were evaluated using alizarin red and the von kossa staining and analyzed by one-way anova followed by tukey’s post hoc test. results: the n-ha/calcium sulfate (cs) mixture significantly promoted cell growth in comparison to pure cs. moreover, addition of rhbmp2 to cs (p=0.02) and also mixing cs with n-ha led to further increase in extracellular calcium production and alp activity (p=0.03). conclusion: this in vitro study indicates that a scaffold material in combination with an osteoinductive material is effective for bone matrix formation.