Evaluation of Cellular Toxicity of Three Denture Base Acrylic Resins

Objective: this study aimed to evaluate the cellular toxicity of two newly-released acrylic resins (futura gen and gc reline hard) in comparison with the conventional heat-cure resin (meliodent).materials and methods: sample discs from each acrylic resin were placed in 24-well culture plates along with l929 mouse fibroblast cell line. a mixture of the rpmi 1640 medium, antibiotics and 10% fbs was added to the plates and the specimens were incubated in a co2 incubator. the amount of light absorption by each plate was determined after 1 hour, 24 hours and 1 week by the mtt assay and elisa. the cytotoxic effect of the resins was compared among groups using the two-way anova and further paired comparison was performed using the post-hoc tukey’s test.results: after 1 hour, futura gen displayed a significantly lower level of light absorption in comparison with meliodent (p=0.03). after 24 hours, gc reline hard rendered a significantly lower level of light absorption compared to melio-dent (p=0.02). after one-week of incubation, the mean absorption rates for gc reline hard, meliodent and futura gen were relatively similar (p>0.05). the lowest and highest level of cytotoxicity among all resins were observed after one week and 1 hour of immersion in water, respectively.conclusion: all the tested resins induced some degree of cytotoxicity. cytotoxic-ity of futura gen, gc reline hard and meliodent resins failed to show any sig-nificant reduction from 24 hours to one week. thus, it is recommended to im-merse the dentures in water for 24 hours prior to delivery to the patient.