Evaluation of the Effect of Plasma Rich in Growth Factors (Prgf) on Bone Regeneration

Objective: reconstruction methods are an essential prerequisite for functional rehabilitation of the stomatognathic system. plasma rich in growth factors (prgf) offers a new and potentially useful adjunct to bone substitute materials in bone reconstructive surgery. this study was carried out to investigate the influ-ence of prgf and fibrin membrane on regeneration of bony defects with and without deproteinized bovine bone mineral (dbbm) on rabbit calvaria. materials and methods: twelve new zealand white rabbits were included in this randomized, blinded, prospective study. four equal 3.3×6.6 mm cranial bone defects were created and immediately grafted with dbbm, prgf+dbbm, prgf+fibrin membrane and no treatment as control. the defects were evaluated with histologic and histomorphometric analysis performed 4 and 8 weeks later. results: adding prgf to dbbm led to increased bone formation as compared with the control group in 4- and 8-week intervals. in dbbm and prgf+fibrin membrane samples, no significant increase was seen compared to the control group. there was also a significant increase in the rate of biodegradation of dbbm particles with the addition of prgf in the 8-week interval. neither noti-ceable foreign body reaction nor any severe inflammation was seen in each of the specimens evaluated. conclusion: under the limitation of this study, adding prgf to dbbm enhanced osteogenesis in rabbit calvarias. applying autologous fibrin membrane in the de-fects was not helpful.