Effect of GBR in Combination with Deproteinized Bovine Bone Mineral on the Healing of Calvarial Defects in Rabbits

Statement of problem: the guided bone regeneration (gbr) technique does not always produce consistent results. bone filling within the space provided by the membrane can be incomplete. purpose: the aim of this study was to evaluate the effect of a collagen membrane (bio-gide) in combination with or without deproteinezed bovine bone mineral (bio-oss) on the healing of calvarial defects in rabbits. materials and methods: twelve new zealand white rabbits were used in this randomized single-blind experimental study. four equal defects were created on the calvarium of all animals. each defect in each rabbit was randomly assigned to one of the following treatment groups: group 1(control), no treatment; group 2, covered with bio-gide; group 3, filled with bio-oss; group 4, filled with bio-oss and bio-gide. the animals were sacrificed for histologic and histomorphometric analysis, 30 and 60 days after treatment. results: a significant difference was not observed in regenerated bone between the control and bio-gide groups (p>0.05), at 1 and 2 months. the amount of regenerated bone was significantly higher (p<0.05) in the bio-oss and bio-oss+bio-gide groups as compared to the control group. the difference in regenerated bone was not significant (p>0.05) between the bio-oss and bio-oss+bio-gide groups. bone regeneration increased significantly in all treatment groups, between the two study periods (p<0.05). conclusion: in groups 3 and 4, the presence of a collagen membrane did not affect the amount of new bone regeneration. according to these results, use of a collagen membrane has no additional benefit in the regeneration of intrabony defects.