the efficacy of different antibiotic compounds in regenerative treatment of immature necrotic teeth

Objectives: endodontic treatment of immature teeth poses a significant challenge, especially in achieving a proper seal using traditional obturation methods. revascularization presents itself as an alternative approach to this problem, and the application of triple antibiotic paste (tap) has been suggested as a means to achieve disinfection during the procedure. this study aims to compare the antibacterial properties of three different antibiotic combinations to assess their effectiveness on root canal disinfection.materials and methods: eighty samples were employed to assess the impact of three antibiotic combinations on enterococcus faecalis, escherichia coli, streptococcus mutans, and a combination thereof. the antibiotics included metronidazole, ciprofloxacin, and cefaclor (ccm), the commonly used tap, and a double antibiotic paste (dap) composed of metronidazole and ciprofloxacin. dentin shavings collected using gates-glidden drills were placed in microtubes containing a 2ml standard bacterial suspension. microtube contents were diluted and cultured on bhi agar plates, with colony counts calculated based on dentine shavings' weight in cfu/mg. kruskal-wallis and dunn’s post-hoc tests were used for statistical analysis and p<0.05 was considered significant.results: a significant difference in mean cfu was observed among all bacterial groups (p<0.05). dunn's post-hoc analysis showed a significant difference only between the control group (methylcellulose) and the other antibiotic groups. there was no significant difference between the other antibiotic groups in two-by-two comparisons.conclusion: there was no significant difference in the antimicrobial properties of dap, tap and ccm. therefore, dap and ccm may be used during regenerative treatment.