effect of casein phosphopeptide amorphous calcium phosphate conditioning on microtensile bond strength of three adhesive systems to deep dentin

Objectives: this study aimed to assess the effect of casein phosphopeptide amorphous calcium phosphate (cpp-acp) on microtensile bond strength of three adhesive systems to deep dentin. materials and methods: in this in vitro study, the occlusal surface of 30 sound human third molars was sectioned at 2 mm below and above the cementoenamel junction. the samples were randomly divided into three groups of optibond solo plus, g-bond, and clearfil se bond. before composite resin bonding, each group was divided into two subgroups with and without dentin preparation with cpp-acp. the teeth were then sectioned into 1 mm rods (n=15), and their microtensile bond strength was measured at a crosshead speed of 0.5 mm/min. data were analyzed using two-way anova and post-hoc bonferroni test. results: there was no significant difference in bond strength of optibond solo plus (p=0.44) and clearfil se bond (p=0.67) with/without cpp-acp. a significant difference was found between the two subgroups of g-bond in this respect (p<0.001). the differences in microtensile bond strength among all subgroups were significant (p<0.05). conclusion: the microtensile bond strength in use of g-bond is significantly higher following dentin treatment with cpp-acp compared with no use of cpp-acp.