validity of selected wbc differentiation flags in sysmex xt-1800i

Background: automatic cell counter devises make the cbc differential very easy and delivering the results in few second. however, the problem with this device is facing a flag requires a time-consuming microscopic review of the specimen which causes unacceptable wait times for patient as well as costs for laboratories. in this study, we calculated the validity of wbc diff flags in sysmex xt-1800i. in addition, we verified the correlation between manual and automated samples. methods: overall, 1095 flagged samples were selected in the period of 6 weeks (imam hospital complex, tehran iran, 2014). the results of both automated and manual counting of the samples were carefully studied and compared. totally, 624 nrbc flags, 450 blast flags, 155 abnormal wbc scatter gram flags, 140 eosinophilia flags and 468 monocytosis flags were identified. results :considering nrbc and blast flags there was a significant difference between our manual counted and automated counted nrbcs and blasts (p<0.05). there was no significant difference between automated and manual counting of flags for wbc scatter gram. a significant difference between automated and manual counting data in flags, eosinophilia and monocytosis was foun (p<0.05). conclusion: maspin expression was reduced in samples with grade ii& iii of invasive ductal carcinoma. based on expression of maspin inc-erb-b2, it seems that more expression happened in normal group comparing with different scores of it. we could suggest that there was a reverse relationship between tumor formation and maspin gene expression. these results showed possible role of maspin as prognostic factor