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expression pattern of telomerase reverse transcriptase (htert) variants and bcl-2 in peripheral lymphocytes of systemic lupus erythematosus patients
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نویسنده
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attar armin ,khosravi maharlooei mohsen ,nazarinia mohammad ali ,hosseini ahmad ,bajalli zohre ,moeini yalda sadat ,monabati ahmad ,amirmoezi fatemeh ,jaberipour mansooreh ,habibagahi mojtaba
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منبع
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iranian journal of pathology - 2020 - دوره : 15 - شماره : 3 - صفحه:225 -231
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چکیده
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Background & objective: it is not clear whether activated lymphocytes of patients with systemic lupus erythematosus (sle) are more proliferative or less apoptotic. we aimed to delineate potential differences between b and t cells of sle patients compared to healthy controls regarding the telomerase activity and apoptosis status. methods: in this cross-sectional case control study, blood samples were taken from 10 sle patients and 10 healthy controls. b and t cells were separated using magnetic cell sorting system. telomeric repeat amplification protocol (trap) assay and real-time pcr were used to determine the telomerase activity and the expression of alternatively spliced variants. results: four patients under treatment showed significant telomerase activity in their t cells. four of the newly diagnosed patients showed telomerase activity in their b cells (20% of all patients and 40% of new onset patients). there was no specific pattern of human telomerase reverse transcriptase variant expression within the patients’ lymphocytes. a significantly reduced expression of bcl-2 was detected in b cells (p=0.018) and a trend toward lower bcl-2 expression in t cells was seen in sle patients compared to healthy controls. conclusion: although not definitive, our results may suggest that b cells may have more active roles during the earlier phases of the disease attack, while t cells take over when the disease reaches its chronic stages.
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کلیدواژه
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apoptosis ,lymphocyte ,systemic lupus erythematosus ,telomerase
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آدرس
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shiraz university of medical sciences, department of cardiovascular medicine, division of interventional cardiology, iran, shiraz university of medical sciences, students’ research committee, cell and molecular medicine research group, iran, shiraz university of medical sciences, internal medicine department, rheumatology division, iran, shiraz university of medical sciences, institute of cancer research, iran, shiraz university of medical sciences, students’ research committee, cell and molecular medicine research group, iran, shiraz university of medical sciences, department of anesthesiology, division of intensive care unit, iran, shiraz university of medical sciences, hematology research center, department of pathology, iran, shiraz university of medical sciences, department of pathology, iran, shiraz university of medical sciences, institute of cancer research, iran, shiraz university of medical sciences, department of immunology, iran
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پست الکترونیکی
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habibagahim@sums.ac.ir
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Authors
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