evaluating the effect of intra-articular injection for bone marrow mesenchymal stem cells in repairing damaged cartilage of rat knee joint

Background: damage in cartilage tissue is one of the global challenges and affects a large percentage of people. trauma or age-related degenerative disease is the main cause of osteoarthritis. the aim of this study was to evaluate the effect of intra-articular injection for bone marrow mesenchymal stem cells and the resulting secretions in repairing the damaged cartilage of the rat knee joint.methods: 30 male rats that weights approximately 200 to 250 g were randomly divided into five groups of 6 rats, including healthy group (receiving physiological serum), osteoarthritis group (monosodium iodoacetate injection), mesenchymal group, mesenchymal group + receiving the same volume of cell secretions + culture medium and culture medium injection group. four weeks later, the rats have been killed and the hip joint examined by both macroscopic and microscopic methods.results: the results showed that the injection of 2.5*105 cells per ml had a good effect on the repairing of cartilaginous lesions caused by the monosodium iodoacetate injection. histological studies showed that fibrillation rate, the presence of small gaps and deep gaps (p <0.05) decreased in the mesenchymal treatment group. the level of proteoglycans in mesenchymal treatment group increased significantly (p <0.05). also, in the treatment group with mesenchymal secretions and culture medium, changes were observed in repairing the damaged cartilage, which was not significantly different from the osteoarthritis group.conclusion: in this study, the restorative effect has benn confirmed for bone marrow mesenchymal stem cells and their secretions in improving histopathological lesions of rat articular cartilage.

evaluating the effect of intra-articular injection for bone marrow mesenchymal stem cells in repairing damaged cartilage of rat knee joint

background: damage in cartilage tissue is one of the global challenges and affects a large percentage of people. trauma or age-related degenerative disease is the main cause of osteoarthritis. the aim of this study was to evaluate the effect of intra-articular injection for bone marrow mesenchymal stem cells and the resulting secretions in repairing the damaged cartilage of the rat knee joint.methods: 30 male rats that weights approximately 200 to 250 g were randomly divided into five groups of 6 rats, including healthy group (receiving physiological serum), osteoarthritis group (monosodium iodoacetate injection), mesenchymal group, mesenchymal group + receiving the same volume of cell secretions + culture medium and culture medium injection group. four weeks later, the rats have been killed and the hip joint examined by both macroscopic and microscopic methods.results: the results showed that the injection of 2.5*105 cells per ml had a good effect on the repairing of cartilaginous lesions caused by the monosodium iodoacetate injection. histological studies showed that fibrillation rate, the presence of small gaps and deep gaps (p <0.05) decreased in the mesenchymal treatment group. the level of proteoglycans in mesenchymal treatment group increased significantly (p <0.05). also, in the treatment group with mesenchymal secretions and culture medium, changes were observed in repairing the damaged cartilage, which was not significantly different from the osteoarthritis group.conclusion: in this study, the restorative effect has benn confirmed for bone marrow mesenchymal stem cells and their secretions in improving histopathological lesions of rat articular cartilage.