In vitro plant regeneration of Iraqi cotton (Gossypium hirsutum L.) cultivars through embryonic axis

In plant tissue culture studies, obtaining new plantlets from different parts of plant is a very important feature with direct or indirect ways of regeneration. the plant show different regeneration capacities from species to species. in this present study, in-vitro direct shoots development from embryonic axis of cotton (gossypium hirsutum l. cv. lashata) were comparatively studied. embryonic axis, embryonic hypocotyl and plumule obtained from five days old in-vitro grown seeds. all explants were cultured on ms media supplemented with 1- 4 mg/l benzyl amino purine (bap) + 0.1 mg/l naphthalene acetic acid (naa) and 3 mg/l activated charcoal. cultured explants were kept at growth chamber with photoperiod for 16 hours light and 8 hours dark, at 25oc. reproducible in-vitro plant regeneration was obtained from embryonic axis when cultured onto ms medium supplemented 1 mg/l bap + 0.1 mg/l naa and 3 g/l activated charcoal. shoot induction was to be noted 53.3%, 36.7% and 12.3 in embryonic axis, plumule and embryonic hypocotyl respectively. all these shoots were capable of rooting on ms medium supplemented with 0.1 mg/l naa, and establishing in soil 3-4 weeks