Influence of various levels of L-asparaginase II purification on the cytotoxicity, DNA level, and apoptosis in Hep-2 cells

The genetic effects of several concentrations of l–asparaginase ii (asnase ii), produced by proteus vulgaris strain pv.u92, at various levels of purification (ultrasonication, precipitation, ion- exchange chromatography and gel filtration chromatography) on cancer cells line of hep–2 were studied. this bacterial enzyme with concentration 4 u/ml at gel filtration level was revealed a putative cytotoxicity against cancer cells in comparison with other concentrations and steps of purification were used in this work. moreover, 4 u/ml of asnase ii at gel purification level has a distinguished role on arrest cancer cells division of hep–2; it was reduced the content of dna at each phase of cancer cell cycle particularly at g2/m phase, the level of dna was 3%. on the other hand, the partial purified enzyme, l–asnase ii, was induced apoptosis by both levels of purification ion– exchange and gel filtration, the apoptotic fractionation was 0.86 and 0.7 respectively.