The effect of local injection of mesenchymal stem cells and IL-10 with platelet lysate on fracture healing in a rat model

Objective: this study investigates the effect of mesenchymal stem cells (msc),an immuneprivileged cell source for tissue repair,interluken (il-10),an immune inhibitory factor,and platelet lysate (pl) on the fracture healing process. methods: forty adult male sprague-dawley rats were anesthetized,and left tibia mid shaft fractures were created. the rats were randomly assigned to 5 groups and received injections with saline ( a); msc and pl (b); il-10 and pl (c); pl (d); and msc,pl,and il-10 (e). lateral radiographs of four randomly selected rats in each group were taken at different days post-fracture. four rats from each group were sacrificed at day twenty one post-fracture,with left tibiae harvested for histological analysis. results: at 7 days post-fracture,the highest level of callus formation and bone healing was observed in group e (treated with msc,pl,and il-10) followed by groups b (treated with msc and pl). the same results were obtained at days 14,21 and 28 post-fracture. at 21days post-fracture,histological analysis revealed that the callus of fractured tibiae in group a consisted mostly of chondrocytes,while in groups c and d,extensive remodeling with a limited amount of chondrocytes present at the fracture. combination of msc,pl,and il-10was associated with the greatest fracture healing performance. after 3 weeks of treatment in group e and b,trabecular bone formation and cortex remodeling were observed. conclusions: the use of msc in combination with il-10 and pl is a promising alternative treatment to promote fracture healing. © 2015 dar publishers ? the university of jordan. all rights reserved.