Effect of Synthetic Tissue Fluid on Microleakage of Grey and White Mineral Trioxide Aggregate as Root-End Filling Materials An in vitro study

Abstract: objectives: the success of endodontic surgery has been shown to depend partly on the apical seal. grey mineral trioxide aggregate (gmta) produces hydroxyapatite twice as often as white mineral trioxide aggregate (wmta) when suspended in a phosphate buffered saline (pbs) solution. the aim of this in vitro study was to compare the microleakage phenomenon of gray and white mineral trioxide aggregates as root-end filling materials after immersion in synthetic tissue fluid (stf). methods: 55 single-rooted extracted maxillary anterior human teeth were divided into two experimental groups of 20 teeth each, plus 3 groups of 5 teeth each as two negative and one positive control groups. the root canals were cleaned, shaped, and laterally compacted with gutta-percha. the root ends were resected and 3 mm deep cavities were prepared. the root-end preparations were filled with gmta or wmta in the experimental groups. leakage was determined using a dye penetration method. data were analysed using analysis of variance (anova) at the 0.05 level of significance. results: the mean dye leakage was 0.40 ± 0.1 mm for gmta and 0.50±0.1 mm for wmta groups, respectively. there was no significant difference between the two experimental groups (p = 0.14). conclusion: despite the different properties and behaviours of gmta and wmta in stf, there were no significant differences in microleakage when using gmta or wmta