antimicrobial efficacy and chemical constituents of pseudostem essential oils from zingiber castaneum

In this paper, chemical constituents and antimicrobial activity of essential oil from the pseudo-stem of zingiber castaneum škorničk. & q.b. (zingiberaceae) nguyễn growing in vietnam havw been reported. essential oils were obtained by hydrodistillation using the clevenger-type apparatus. chemical components of the essential oil were analyzed by gas chromatography (gc) and gas chromatography coupled with mass spectrometry (gc/ms). the minimum inhibitory concentrations (mic) were evaluated by the method of microdilution broth susceptibility assay. the main constituents of the oil were bicyclogermacrene (28, 15.8%), cis-β-elemene (18, 9.8%) and germacrene d (26, 9.2%). the pseudo-stem oil of z. castaneum displayed antimicrobial activity against pseudomonas aeruginosa (atcc 25923), aspergillus niger (atcc 9763) and fusarium oxysporum (atcc 48112) with mic values of 12.5 ± 0.57 μg/ml, 50 ± 1.00 μg/ml and 50 ± 0.50 μg/ml, respectively. the results indicate the potential of z. castaneum essential oil as a source of antimicrobial agent.