phytochemical study and antiinflammatory activity of the roots of mangifera indica l. in lipopolysaccharide (lps)stimulated peritoneal macrophages

This study deals with the identification of secondary metabolites of methanol extract of the roots of mangifera indica l and the assessment of plant in vitro antiinflammatory activity. highperformance liquid chromatography with electrospray ionization mass spectrometric detection (hplcesimsn) using the negative ion mode was performed to establish the chromatographic fingerprint and identify various chemical components of the plant extract. the antiinflammatory effect of the meoh extract (3, 30 and 300 μg/ml) was assessed through cell viability and nitric oxide (no) production on nonstimulated and lpsstimulated peritoneal macrophages. phytochemical analysis indicated the presence of a number of phenolic compounds where galloyl derivatives, mangiferin and its derivatives were the major constituents. the methanol extract exhibited significant concentrationdependent inhibitory effect on no production, both on stimulated and nonstimulated macrophages. the concentration 300 μg/ml showed significant cell toxicity. the methanol extract of mangifera indica is rich in phenolic compounds and possesses potent in vitro antiinflammatory activity, but its higher concentrations are cytotoxic.