phytoconstituents profiling of cissus rotundifolia (forssk.) vahl. by hplc-ms/ms, and evaluation of its free radical scavenging activity (dpph) and cytotoxicity

Characterization of phytoconstituents in the methanolic extract (70%) of cissus rotundifolia (forssk.) vahl. was performed using high-performance liquid chromatography (hplc) coupled with electrospray ionization mass spectrometry (eims). furthermore, tandem mass spectrometry (ms/ms) was performed to assist the structural elucidation of compounds. accordingly, twenty-seven compounds were identified involving four acids, sixteen phenolics, two steroidal saponins, two coumarins, two stilbenoids and one triterpene. astragalin 1, β-amyrin 2 and 1β-hydroxy-kryptogenin-1-o-α-l-rhamnopyranosyl-(1→2)- α-l-arabinopyranoside 3 were isolated by column chromatography and their structures were identified by spectral analysis including 1d and 2d nmr. the plant extract and the isolated compounds 1-3 were evaluated for their radical scavenging activity. compounds 1 and 2 showed significant activity relative to that of ascorbic acid as a standard. the cytotoxicity of the plant extract, the isolated compounds and the standard doxorubicin were evaluated using the three human tumor cell lines ht116, mcf-7 and pc-3. in this regard, the plant extract exhibited significant cytotoxicity against mcf-7 while no activity was observed against the other cell lines. the isolated compounds 1-3 showed no activity against the used cell lines, as well.