Equine Bone Marrow Derived Mesenchymal Stem Cells: Isolation and Multilineage Differentiation

Objective- to evaluate growth characteristics and differentiation capacity of equine mesenchymal stem cell (emscs) derived from bone marrow (bm). study design- in vitro experimental study. animals- four young adult horses (2-5 years old) procedure- cell morphology and growth characteristics of emscs harvested from bm were evaluated in standard culture conditions. emscs in passage 3 were subjected to osteogenic and adipogenic differentiation induction to investigate their differentiation potential.pcr analysis of differentiated cells was done to determine differentiation. results-the cells expanded to sufficient quantity for therapeutic purposes within days and they survived to later passages while sustained their fibroblast-like morphology. positive osteogenesis was detected via alizarin red staining and adipogenesis was confirmed by oil red o staining. the cells were more potential to differentiate into osteoblasts rather than adipocytes. pcr analysis approve relative expression of osteogenic and adipogenic genes. conclusions and clinical relevance - the results of this study further support ebm-mscs as a cell population with the capacity to proliferate and differentiate down the osteogenic and adipogenic lineages. equine bm is a potentials source of mscs for cell-based regenerative therapies in horse.