Effects of Hydrocortisone on the Growth of Cultured Equine Fibroblasts Isolated From Distal Aspects of the Limb

Objective- to determine the effects of the hydrocortisone on in vitro growth of fibrobla sts isolatedfrom the distal aspects of the horses limbs.design- experimental in vitro study.animals- a total of 4 caspian miniature and 4 mixed thoroughbred horses. procedures- under generalanesthesia and aseptic condition, a full thickness of skin incision was created on the lateral aspects of themetacarpal mid-third and 3 grams of subcutaneous tissue was harvested and placed on culture medium(rpmi-1640) in an incubator at 37°c in 5%c02. the hydrocortisone was added in 3 different concentrations [treatm ent groups a (l014 g/ml) , b (100 14g/ml), and c (300 14g/ml)] into the culture medium and cellcounting was carried out after 4 days. the effects of hydrocortisone added to control media were comparedwith a control media on fibroblast growth and viability. the data were analyzed by one way anova andtukey test.results- there were no significant differences in fibroblast growth rate and viability percentage (p>o.05)between the groups control and a, but the groups band c suppressed significantly the fibroblast growthand decreased viability percentage in compared with groups control and a (p<0.05), and also there were nosignificant differences between groups band c in fibroblast growth rate and viability percentage (p>0.05).conclusion and clinical relevance- the effect of hydrocortisone on cultured fibroblast growth isdose-dependant. application ofthis agent can inhibit fibroblast growth; therefore it can be used for treatmentofexuberant granulation tissue and improving wound healing processes, especially in the distal limb woundsof the horses.