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   Effects of Hydrocortisone on the Growth of Cultured Equine Fibroblasts Isolated From Distal Aspects of the Limb  
   
نویسنده Azari Omid ,Ghamsari Mehdi ,Dehghan Mohammad Mehdi ,Aghcheloo Mohammad Reza
منبع Iranian Journal Of Veterinary Surgery - 2006 - دوره : 1 - شماره : 1 - صفحه:7 -14
چکیده    Objective- to determine the effects of the hydrocortisone on in vitro growth of fibrobla sts isolatedfrom the distal aspects of the horses limbs.design- experimental in vitro study.animals- a total of 4 caspian miniature and 4 mixed thoroughbred horses. procedures- under generalanesthesia and aseptic condition, a full thickness of skin incision was created on the lateral aspects of themetacarpal mid-third and 3 grams of subcutaneous tissue was harvested and placed on culture medium(rpmi-1640) in an incubator at 37°c in 5%c02. the hydrocortisone was added in 3 different concentrations [treatm ent groups a (l014 g/ml) , b (100 14g/ml), and c (300 14g/ml)] into the culture medium and cellcounting was carried out after 4 days. the effects of hydrocortisone added to control media were comparedwith a control media on fibroblast growth and viability. the data were analyzed by one way anova andtukey test.results- there were no significant differences in fibroblast growth rate and viability percentage (p>o.05)between the groups control and a, but the groups band c suppressed significantly the fibroblast growthand decreased viability percentage in compared with groups control and a (p<0.05), and also there were nosignificant differences between groups band c in fibroblast growth rate and viability percentage (p>0.05).conclusion and clinical relevance- the effect of hydrocortisone on cultured fibroblast growth isdose-dependant. application ofthis agent can inhibit fibroblast growth; therefore it can be used for treatmentofexuberant granulation tissue and improving wound healing processes, especially in the distal limb woundsof the horses.
کلیدواژه Hydrocortisone ,Fibroblast ,Growth ,Horse
آدرس University Of Tehran, Faculty Of Veterinary Medicine, Department Ofclinical Sciences, ایران, University Of Tehran, Faculty Of Veterinary Medicine, Department Ofclinical Sciences, ایران, University Of Tehran, Faculty Of Veterinary Medicine, Department Of Clinical Science, ایران, University Of Tehran, Faculty Of Veterinary Medicine, Department Ofclinical Sciences, ایران
 
 

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