Effect of Heparin in Prevention of Experimentalabdominal Adhesions in Rat

Study on the effect of heparin in prevention of abdominal adhesions followingabdominal surgery in rat.design- experimental design.animals-twenty male rats with average weight of 248 ± 5 gr.procedures- animals were randomly divided into two equal groups of 10 rats (a and b).after induction of anesthesia, a midline abdominal incision was made and intestines weremanipulated with a standard method, using a mini johns hopkins bulldog clamp. in groupa (treatment) 100u/kg of heparin was administered into the peritoneal cavity and in groupb (control) the same volume of saline was administered intrapritoenally. the abdominalincision was closed routinely. in group a 40 u/kg heparin and in group b the same volumeof normal saline was injected subcutaneously bid. seven days after surgery, all rats weresacrificed for necropsy. the number and the location of adhesions were evaluated andtissue samples were taken for histopathological examination.results- macroscopic inspection revealed much more adhesions in group b compared withgroup a. in histopathological sections, the granulation tissue in treatment group wasmainly “early” and the number of neutrophils and fibrin formation was less than the controlgroup.conclusion and clinical relevance- heparin treated rats showed less fibrinous adhesionin macroscopic studies and microscopic slides. regarding to the results of current study,use of heparin can reduce peritoneal adhesion in peritonitis and can be used after majorsurgeries in rat.