Pathological Evaluation of Two Techniques of Amniotic Membrane Patching on Experimentally Induced Corneal Lesions in Dogs

Objective- this study was performed to evaluate and compare grossly and pathologicallytwo surgical techniques of amniotic membrane patching on experimental corneal alkali burnin dogs.design- experimental study.animals- fifteen healthy dogsprocedures- the central corneas of the dogs were inflicted by applying a round filter paper,6.0 mm in diameter, soaked in 1 n naoh for 60 sec. a corneal alkali burn was madeunilaterally in each dog's eye. a total of fifteen dogs were divided into three groups of fiveeach: (a) uncovered as a control group; (b) covered by amniotic membrane (am) patchingwith the amnion cell side down sutured around the corneal wound area with 10/0 nylon asam+suture group; (c) covered by sutureless amniotic membrane patch adhered by 2-octylcyanoacrylate (dermabond®) as am+glue group. time of surgery and am persistence were recorded. after 3 weeks eyes enucleated and samples were monitored by evaluating of mostimportant pathological features.results- the mean time of surgery in group b was significantly longer than group c. ampersistence in group c was significantly more than group b. corneal thickness measurementsand numbers of epithelial rows showed no significant statistical differences between differentgroups. group c has had significant lower degree of corneal vascularization than group a.stromal edema was most prominent in group a, but statistical analysis revealed nosignificant statistical differences between groups. stromal collagen arrangement showed nosignificant differences. am patched corneas (group b, c) show lower inflammation,compared with the uncovered one (group a). among the am patching groups, the group crevealed the best result.conclusion and clinical relevance- use of the sutureless amniotic membrane patching with2-octyl cyanoacrylate as a dressing on a corneal alkali burn could induce rapid epithelialhealing with less inflammatory response as well as reduce the time of operation in dogs.