Growth Kinetics and in Vitro Aging of Mesenchymal Stem Cells Isolated From Rat Adipose Versus Bone Marrow Tissues

Objective- to investigate and compare growth potential as well as aging of mesenchymalstem cells (mscs) derived from rat bone marrow tissue and adipose tissue (at) occurred atepicardial and epididymal regions.design- experimental study.animals- 10 wistar rats.procedures- rat mscs occurred at bone marrow and epicardial and epididymal at wereisolated and culture expanded through several successive passages. differentiation potentialalong bone, cartilage and adipose cell lineages was used to verify msc identity of theisolated cells, and then the cells were comparatively investigated in terms of theircolonogenic ability, population doubling time and growth curve characteristics. furthermore,the number of senescent cells at different passages was quantified using senescent- associated(sa) beta galactosidase staining method.results- mscs from both at appeared to have more proliferation capacity in culture thanthose from bone marrow since they exhibited significantly more colony number and shorterpdt value (p<0.05). epicardial at-mscs indicated even more significant proliferationcapacity than their epididymal counterparts. with respect to cell aging, marrow-mscscultures indicated higher percentages of senescent cells than at-mscs (p<0.05). althoughthe percentages of senescent cells in epididmal at-mscs were higher than epicardial atmscsbut the difference was not statistically significant.conclusion and clinical relevance- taken together we concluded that rat epicardial atmscscould be appropriate cells for experimental and preclinical settings since they possessmore expansion rate and less percentages of senescent cells in culture.