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Isolation, Identification of Lactobacillus mucosae AN1 and its Antilisterial Peptide Purification and Characterization
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نویسنده
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Ayyanna Repally ,Venkatesh Perumal ,Ankaiah Dasari ,Esakkiraj Palanichamy ,Arul Venkatesan
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منبع
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probiotics and antimicrobial proteins - 2018 - دوره : 10 - شماره : 4 - صفحه:775 -786
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چکیده
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lactobacillus mucosae strain an1 isolated from sheep milk and characterized for its probiotic suitability. in vitro evaluation of critical gut endurance properties of this strain were assessed by different screening methods such as bile salt, gastric acid, lysozyme tolerance assays, hemolytic, cholesterol reduction properties, and ht-29 cell line adhesion assay. antibacterial peptide from this strain was purified using ammonium sulphate precipitation, gel filtration chromatography and reverse-phase hplc. the molecular mass of peptides was determined by tricine-sds-page and confirmed by matrix-assisted laser desorption ionization-time of flight mass spectroscopy (maldi-tof-ms). purified peptide was named as an1 having a molecular mass of 10.66 kda. helical structures of peptide were determined using circular dichroism spectroscopy. stability of peptide an1 towards different parameters such as ph, temperature, organic solvents, proteolytic, and glycolytic enzymes was also analyzed.
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کلیدواژه
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Sheep milk ,L. mucosae AN1 ,Beneficial bacteria ,Antilisterial property ,Food preservation ,Agri-food industry
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آدرس
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Pondicherry University, Department of Biotechnology, India, Pondicherry University, Department of Biotechnology, India, Pondicherry University, Department of Biotechnology, India, Pondicherry University, Department of Biotechnology, India, Pondicherry University, Department of Biotechnology, India
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Authors
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