Ultra-Sensitive Electrochemical On-Line Determination of Clarithromycin Based on Poly(L-Aspartic Acid)/Graphite Oxide/Pristine Graphene/Glassy Carbon Electrode

In this work, a novel and extra sensitive method for on-line monitoring of clarithromycin in the whole blood sample was introduced based on coupling of electro-membrane extraction (eme) and stripping fast fourier transform continuous cyclic voltammetry (sfftccv). in this method, the potential waveform was continuously applied on a poly(l-aspartic acid)/graphite oxide/pristine graphene/glassy carbon electrode and the electrode response was obtained by detracting the background current and also in the following integration with the current one in the specific potential range of oxidation of the analyte. this method was performed by applying a dc potential and migration of clarithromycin from the sample solution into a layer of 4-methyl-2-pentanol that is immobilized in the pores of the sheet membrane and then migrated into the acceptor solution. a low and valuable detection limit of 1.0 ng ml-1 and quantification limit of 6.0 ng ml-1are considered as a part of the sensible results of this experiment. furthermore, an efficient linearity in the range of 6.0-1000 ng ml-1 was found.