reverse flow injection method for estimation of hesperidin and naringenin in supplements with 3-methyl-2-benzothiazolinone hydrazone hydrochloride

The detection of hesperidin (hsp) and naringenin (nar) in dietary supplements and human urine samples is recommended using a reversed flow-injection analysis (rfia) technology that is quick, accurate, and highly responsive. using 3-methyl-2-benzothiazolinone hydrazone hydrochloride (mbth) in the presence of sodium periodate as an oxidizing agent, the suggested approach uses oxidative coupling procedures. with a peak absorbance for hsp at 507 nm and for nar at 495 nm, a red complex is the resultant product. an extensive analysis of the physical and chemical elements influencing the reaction has been conducted. between 2 and 150 µg/ml, the calibration curve showed a steady and proportionate relationship. it was found that the detection limits for nar and hsp were 0.911 µg/ml and 0.856 µg/ml, respectively. for hsp and nar, the relative standard deviation (rsd) was found to be 0.79% and 0.64%, respectively. drug quantitation was effectively accomplished using the recommended methods.