Optimization and modeling of growth conditions of Lactobacillus brevis IBRC10818 for biosynthesis of gamma aminobutyric acid, affected by ultrasonic shock

Gamma-aminobutyric acid (gaba) is a crucial inhibitory neurotransmitter in the nervous system. microbial biosynthesis of gaba has gained attention as an effective production method. this study aimed to optimize and model the production of gaba from lactobacillus brevis ibrc 10818 using an ultrasonic shock and response surface methodology. the factors investigated included ultrasonic intensity, ultrasonic time, incubation period, and monosodium glutamate concentration. the shock was applied during the lag phase of growth. the highest predicted gaba production for l. brevis ibrc10818 was 212.2 mg/l, with optimal conditions of 2.98% monosodium glutamate, 70.8 hours of incubation, 6.30 minutes of shock time, and a frequency of 33 khz. three replicates were conducted under these optimal conditions, resulting in an actual gaba production of 203.5 mg/l, which closely matched the predicted value. this study demonstrates the successful optimization of growth conditions and validates the accuracy of the model.