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   comparison of the culture and pcr methods to determine th prevalence and antibiotic resistance of helicobacter pullorum isolated from chicken thigh samples in semnan, iran  
   
نویسنده jebellijavan ashkan ,emadi chashmi hesamodin ,staji hamid ,akhlaghi hossein
منبع journal of human, environment and health promotion - 2020 - دوره : 6 - شماره : 4 - صفحه:167 -172
چکیده    background: helicobacter pullorum is among the most frequently reported pathogens in poultry. the present study aimed to compare the culture and polymerase chain reaction (pcr) methods to assess the prevalence of h. pullorum isolated from chicken tight samples in semnan, iran. the antibiotic resistance pattern of the h. pullorum isolates was also determined for the first time in iran.methods: in total, 50 chicken thigh samples were collected from the local retail markets in semnan city during januaryseptember 2019. the samples were examined using the culture method and biochemical tests, and the final confirmation was based on pcr with the 16s rrna gene. in addition, antibiotic resistance test was performed using the discdiffusion method.results: according to the culture method and biochemical tests, 12 samples (24%) were positive for h. pullorum, eight of which (16%) showed h. pullorum in pcr as well and resistance against nalidixic acid and ciprofloxacin. on the other hand, the lowest antimicrobial resistance was observed against colistin, chloramphenicol, and fosfomycin.conclusion: our promising findings indicated that pcr based on the 16s rrna gene may be a valuable and sensitive approach to the detection of h. pullorum in chicken meat.
کلیدواژه h.pullorum ,chicken thigh ,pcr test ,culture method ,antibiotic resistance
آدرس semnan university, faculty of veterinary medicine, department of food hygiene and quality control, iran, semnan university, faculty of veterinary medicine, department of clinical science, iran, semnan university, faculty of veterinary medicine, department of pathobiology, iran, semnan university, faculty of veterinary medicine, iran
پست الکترونیکی hoseinakhlaghi599@gmail.com
 
 

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