>
Fa   |   Ar   |   En
   Biochemical and Molecular Identification of Listeria Monocytogenes and Escherichia Coli in the Raw Milk Samples Delivered To the Dairy Farms in Golestan Province, Iran  
   
نویسنده Rezaei Marziyeh ,Khomeiri Morteza ,Ebrahimi Maryam ,Kiani Sonia ,Raeisi Mojtaba
منبع Journal Of Human Environment And Health Promotion - 2019 - دوره : 5 - شماره : 4 - صفحه:160 -164
چکیده    Background: milk is an essential human nutrient, and the monitoring of its sanitation is vital during transportation and storage. the present study aimed to assess bacteriological contamination with escherichia coli and listeria monocytogenes in the raw milk samples of the dairy farms in golestan province, iran. methods: in total, 100 samples were collected from dairy farms in hot and cold seasons. the frequency of e. coli and l. monocytogenes was determined using biochemical tests and polymerase chain reaction (pcr). results: the biochemical tests indicated that 28% and 27% of the samples were contaminated with e. coli and l. monocytogenes based on the culturedependent methods, respectively. in addition, 35 and 40 samples were contaminated with e. coli and l. monocytogenes based on pcr, respectively. pcr had higher sensitivity compared to the biochemical tests (p ˂ 0.05). e. coli and l. monocytogenes contamination was significantly higher in traditional dairy farms than industrial dairy farms (p ˂ 0.05). however, seasonal sampling and geographical region had no significant effects on the contamination load.conclusion: according to the results, e. coli and l. monocytogenes were highly frequent in the raw milks samples. however, the microbial loads had no significant differences in hot seasons and traditional dairy farms.
کلیدواژه Escherichia Coli ,Listeria Monocytogenes ,Raw Milk ,Polymerase Chain Reaction ,Biochemical Test
آدرس Baharan Higher Education Institute, Department Of Food Science And Engineering, Iran, Gorgan University Of Agricultural Sciences And Natural Resources, Faculty Of Food Technology, Department Of Food Science And Technology, Iran, Gorgan University Of Agricultural Sciences And Natural Resources, Faculty Of Food Technology, Department Of Food Science And Technology, Iran, Baharan Higher Education Institute, Department Of Food Science And Engineering, Iran, Golestan University Of Medical Sciences, Laboratory Science Research Center, Iran
پست الکترونیکی raeisi.mojtaba@yahoo.com
 
     
   
Authors
  
 
 

Copyright 2023
Islamic World Science Citation Center
All Rights Reserved