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   Investigation of Enzyme Immobilization Effects on Its Characteristics  
   
نویسنده Mohammadi Javad ,Mohammadi Mehdi ,Mehrasbi Mohammad Reza ,Peyda Mazyar
منبع Journal Of Human Environment And Health Promotion - 2016 - دوره : 1 - شماره : 4 - صفحه:183 -188
چکیده    Background: enzymes are well known as sensitive catalysts in the laboratory and industrial scale. to improve their properties and for using their significant potential in various reactions as a useful catalyst the stability of enzymes can often require improvement. enzymes immobilization on solid supports such as epoxyfunctionalized ferric silica nanocomposite can be effective way to improve their characteristics. methods: in this study silica coated magnetite nanoparticles were functionalized with gptsm as a linker, then immobilization reaction performed by using various amounts of lipase b from candida antarctica (calb), for the next step immobilization effects on thermal stability and optimum ph were investigated in comparison with free calb. results: results illustrated enzyme was successfully immobilized on nano particles and immobilized derivative retains 100% of its activity by 55°c while free calb loss its activity at the same condition. conclusion: immobilization of calb on fe3o4@sio2 particles resulted in significant improvements in its characteristics such as thermal stability and methanol tolerance compared to the free calb.
کلیدواژه Immobilization ,Candida Antarctica Lipase B ,Silica Nanocomposite Magnetite
آدرس Zanjan University Of Medical Sciences, School Of Public Health, Department Of Environmental Health Engineering, ایران, National Institute Of Genetic Engineering And Biotechnology (Nigeb), ایران, Zanjan University Of Medical Sciences, School Of Public Health, Department Of Environmental Health Engineering, ایران, Zanjan University Of Medical Sciences, School Of Public Health, Department Of Environmental Health Engineering, ایران
پست الکترونیکی mazyarpeyda@zums.ac.ir
 
     
   
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